ANTISERUM AGAINST RAT LUTEINIZING-HORMONE (LH) RECEPTORS

被引：17

作者：

LUBORSKY, JL ^{[1
]}

BEHRMAN, HR ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT PHARMACOL,NEW HAVEN,CT 06510

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(79)91192-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An antiserum to soluble rat luteal LH receptors was produced in rabbits. The presence of LH receptor antibodies was shown by indirect immuno-precipitation of [125]hCG-soluble rat LH receptor-receptor antiserum complexes by antirabbit IgG. The antiserum bound LH receptors from rat ovaries and testes but not those from human or sheep ovaries. Unoccupied LH receptors competed with [125I]hCG occupied receptors in a dose-dependent fashion for antiserum binding. Extracts of rat brain and liver did not appear to bind to the antiserum. In addition, LH-dependent progesterone secretion from isolated rat ovarian cells was reduced by the antiserum although there was no change in hormone binding. © 1979.

引用

收藏

页码：1407 / 1413

页数：7

相关论文

共 23 条

[11] MODULATION OF ACETYLCHOLINE-RECEPTOR BY ANTIBODY AGAINST RECEPTOR [J].

HEINEMANN, S ;

BEVAN, S ;

KULLBERG, R ;

LINDSTROM, J ;

RICE, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (07) :3090-3094

[12] PGF2-ALPHA-INDUCED LOSS OF CORPUS-LUTEUM GONADOTROPIN RECEPTORS [J].

HICHENS, M ;

GRINWICH, DL ;

BEHRMAN, HR .

PROSTAGLANDINS, 1974, 7 (06) :449-458

[13] ANTIBODIES TO PURIFIED INSULIN RECEPTOR HAVE INSULIN-LIKE ACTIVITY [J].

JACOBS, S ;

CHANG, KJ ;

CUATRECASAS, P .

SCIENCE, 1978, 200 (4347) :1283-1284

[14] BINDING OF ANTIBODIES TO ACETYLCHOLINE RECEPTORS IN ELECTROPHORUS AND TORPEDO ELECTROPLAX MEMBRANES [J].

KARLIN, A ;

HOLTZMAN, E ;

VALDERRAMA, R ;

DAMLE, V ;

HSU, K ;

REYES, F .

JOURNAL OF CELL BIOLOGY, 1978, 76 (03) :577-592

[15]

ORCZYK GP, 1979, METHOD HORM RADIOIMM, P701

[16] STUDIES ON PURIFIED EEL ACETYLCHOLINE-RECEPTOR AND ANTI-ACETYLCHOLINE RECEPTOR ANTIBODY [J].

PATRICK, J ;

LINDSTROM, J ;

CULP, B ;

MCMILLAN, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (12) :3334-3338

[17] PROTEIN HORMONE ACTION - KEY TO UNDERSTANDING OVARIAN FOLLICULAR AND LUTEAL CELL DEVELOPMENT [J].

RICHARDS, JS ;

MIDGLEY, AR .

BIOLOGY OF REPRODUCTION, 1976, 14 (01) :82-94

[18] ROLE OF MEMBRANE-BOUND RECEPTORS [J].

RYAN, RJ ;

LEE, CY .

BIOLOGY OF REPRODUCTION, 1976, 14 (01) :16-29

[19] INTERACTION OF CELL-MEMBRANE PROLACTIN RECEPTOR WITH ITS ANTIBODY [J].

SHIU, RPC ;

FRIESEN, HG .

BIOCHEMICAL JOURNAL, 1976, 157 (03) :619-&

[20] BLOCKADE OF PROLACTIN ACTION BY AN ANTISERUM TO ITS RECEPTORS [J].

SHIU, RPC ;

FRIESEN, HG .

SCIENCE, 1976, 192 (4236) :259-261