A NOVEL NUCLEAR-PROTEIN WHICH BINDS TO G-GAMMA AND A-GAMMA GLOBIN PROMOTERS AND MODULATES HEMOGLOBIN-SYNTHESIS IN K562 CELLS

被引：3

作者：

BALIGA, BS

PHILLIPS, K

ALIABADI, Z

MANKAD, VN

机构：

[1] Molecular Biology Division, Comprehensive Sickle Cell Center, University of South Alabama College of Medicine, Mobile, Alabama

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 1992年 / 49卷 / 04期

关键词：

NUCLEAR PROTEIN; GLOBIN PROMOTERS; K562; CELLS; HEMOGLOBIN; HEMIN;

D O I：

10.1002/jcb.240490409

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear extract of human erythroleukemic cell line K562 contains a 70 kDa protein which is gradually reduced when cells are induced to express globin genes by 25-mu-M hemin. When globin synthesis was inhibited by cycloheximide (100 mu-g/ml) or Actinomycin D (1-mu-g/ml), the disappearance of this protein was prevented. The 70 kDa nuclear protein exhibited strong binding to G-gamma and A-gamma globin promoters but not to beta-globin promoter. This suggests that this 70 kDa nuclear protein may be involved in the regulation of fetal globin gene expression.

引用

页码：394 / 398

页数：5

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