ISOTACHOPHORESIS OF NUCLEIC-ACID CONSTITUENTS

被引：11

作者：

BRUCHELT, G ^{[1
]}

NIETHAMMER, D ^{[1
]}

SCHMIDT, KH ^{[1
]}

机构：

[1] UNIV TUBINGEN,DEPT SURG,W-7400 TUBINGEN 1,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 618卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(93)80027-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Determination methods for purine and pyrimidine bases, nucleosides, nucleotides and related compounds using analytical capillary isotachophoresis are reviewed. First, the isotachophoretic characterization of these compounds, as well as methods for sample preparation prior to analysis, and the different ways of detecting unknown substances in complex biological systems are described. Then applications of isotachophoretic analysis in medical diagnosis and biomedical research are reviewed. In particular, the analysis of purines, pyrimidines, nucleosides and related compounds in blood and serum for the diagnosis and treatment of inherited diseases and cancer is described. Selected applications of nucleotide analysis in biomedical research using different tissue extracts are also reviewed, and some examples of nucleotide-dependent enzymic reactions, which were performed by means of analytical isotachophoresis, are presented.

引用

页码：57 / 77

页数：21

共 103 条

[81] PHARMACOKINETICS OF 5-FLUOROURACIL - INTERRELATIONSHIP WITH BIOCHEMICAL KINETICS IN MONITORING THERAPY [J].