SEXUAL-STAGE-SPECIFIC RNA EXPRESSION OF A NEW PLASMODIUM-FALCIPARUM GENE DETECTED BY IN-SITU HYBRIDIZATION

被引：19

作者：

BAKER, DA

THOMPSON, J

DARAMOLA, OO

CARLTON, JMR

TARGETT, GAT

机构：

[1] UNIV LONDON IMPERIAL COLL SCI & TECHNOL,DEPT BIOL,LONDON SW7 2BB,ENGLAND

[2] UNIV EDINBURGH,DIV BIOL SCI,EDINBURGH EH9 3JT,MIDLOTHIAN,SCOTLAND

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1995年 / 72卷 / 1-2期

基金：

英国惠康基金;

关键词：

PLASMODIUM FALCIPARUM; GAMETOCYTE; MALARIA; IN SITU HYBRIDIZATION;

D O I：

10.1016/0166-6851(95)00073-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene, Pf77, transcribed in the sexual stages of Plasmodium falciparum was isolated from a genomic expression library with a polyclonal antibody raised to gametocyte proteins. The entire coding region was obtained from a series of overlapping genomic and cDNA clones (from gametocyte RNA). A single open reading frame is present with no introns and no tandem repeat sequences. A Pf77 probe hybridised to a single transcript present in RNA prepared from purified gametocytes and could not be detected in RNA prepared from asexual blood stages. In situ hybridisation studies confirmed that the expression of Pf77 mRNA is sexual-stage-specific and in addition, showed that Pf77 mRNA is present only in female gametocytes during the vertebrate stages of the parasite's development.

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页码：193 / 201

页数：9

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