RECONSTITUTION OF TRANSCRIPTION FACTOR SL1 - EXCLUSIVE BINDING OF TBP BY SL1 OR TFIID SUBUNITS

被引：130

作者：

COMAI, L ^{[1
]}

ZOMERDIJK, JCBM ^{[1
]}

BECKMANN, H ^{[1
]}

ZHOU, S ^{[1
]}

ADMON, A ^{[1
]}

TJIAN, R ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY, HOWARD HUGHES MED INST, DEPT MOLEC & CELL BIOL, BERKELEY, CA 94720 USA

来源：

SCIENCE | 1994年 / 266卷 / 5193期

关键词：

D O I：

10.1126/science.7801123

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

RNA polymerase I and II transcription factors SL1 and TFIID, respectively, are composed of the TATA-binding protein (TBP) and a set of TBP-associated factors (TAFs) responsible for promoter recognition. How the universal transcription factor TBP becomes committed to a TFIID or SL1 complex has not been known. Complementary DNAs encoding each of the three TAF(I)s that are integral components of SL1 have now been isolated. Analysis of subunit interactions indicated that the three TAF(I)s can bind individually and specifically to TBP. In addition, these TAF(I)s interact with each other to form a stable TBP-TAF complex. When TBP was bound first by either TAF(I)110, 63, or 48, subunits of TFIID such as TAF(II)250 and 150 did not bind TBP. Conversely, if TBP first formed a complex with TAF(II)250 or 150, the subunits of SL1 did not bind TBP. These results suggest that a mutually exclusive binding specificity for TBP intrinsic to SL1 and TFIID subunits directs the formation of promoter- and RNA polymerase-selective TBP-TAF complexes.

引用

页码：1966 / 1972

页数：7

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