DOWN-REGULATION OF COLLAGEN-SYNTHESIS IN FIBROBLASTS WITHIN 3-DIMENSIONAL COLLAGEN LATTICES INVOLVES TRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL MECHANISMS

被引：79

作者：

ECKES, B ^{[1
]}

MAUCH, C ^{[1
]}

HUPPE, G ^{[1
]}

KRIEG, T ^{[1
]}

机构：

[1] UNIV COLOGNE, DEPT DERMATOL, W-5000 COLOGNE 41, GERMANY

来源：

FEBS LETTERS | 1993年 / 318卷 / 02期

关键词：

GENE EXPRESSION; RNA STABILITY; EXTRACELLULAR MATRIX; FIBROBLAST;

D O I：

10.1016/0014-5793(93)80006-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Culturing human fibroblasts in a three-dimensional collagen matrix leads to a reduction of collagen I by more than 90%, both on the level of mRNA steady-state as well as protein. In order to differentiate changes in de novo transcription and posttranscriptional control, nuclear run on assays and pulse/chase experiments determining mRNA stability were used. Our results indicate that de novo transcription of the COL1A1 gene and pro-alpha1(I)collagen mRNA half-life are both decreased by 50% in fibroblasts grown in three-dimensional collagen lattices as compared to monolayer cultures. The extracellular matrix therefore elicits signals which are transduced from the cell surface to the inside of fibroblasts resulting in a specific reprogramming of transcriptional as well as posttranscriptional processes.

引用

页码：129 / 133

页数：5

共 39 条

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