STRUCTURE AND CHROMOSOMAL LOCALIZATION OF THE HUMAN 2',3'-CYCLIC NUCLEOTIDE 3'-PHOSPHODIESTERASE GENE

被引：12

作者：

DOUGLAS, AJ

FOX, MF

ABBOTT, CM

HINKS, LJ

SHARPE, G

POVEY, S

THOMPSON, RJ

机构：

[1] UNIV SOUTHAMPTON,SOUTHAMPTON GEN HOSP,BIOCHEM CLIN,LEVEL D,S LAB & PATHOL BLOCK,TREMONA RD,SOUTHAMPTON S09 4XY,ENGLAND

[2] UNIV LONDON UNIV COLL,MRC,HUMAN BIOCHEM GENET UNIT,LONDON NW1 2HE,ENGLAND

[3] UNIV LONDON UNIV COLL,DEPT GENET & BIOMETRY,GALTON LAB,LONDON NW1 2HE,ENGLAND

来源：

ANNALS OF HUMAN GENETICS | 1992年 / 56卷

基金：

英国惠康基金;

关键词：

D O I：

10.1111/j.1469-1809.1992.tb01149.x

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Human brain cDNA clones for the myelin associated enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) have been isolated and sequenced. The only 5' untranslated region (UTR) sequence found was that of a human CNPII mRNA, with no direct evidence for a CNPI mRNA. Human CNPase cDNAs were used to isolate genomic clones containing the human CNPase gene which is 9 kb long. Four exons were identified, separated by three introns, and the sequence of each exon and intron/exon boundary has been established. The polymerase chain reaction (PCR) was used to detect the presence of the human CNPase gene in DNA from a panel of rodent/human somatic cell hybrids. By this means the human CNPase gene was mapped to chromosome 17. In situ hybridization of a human CNPase genomic clone to metaphase chromosomes further localized this gene to chromosomal band 17q21.

引用

页码：243 / 254

页数：12

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