NOVEL AFFINITY-BASED PROCESSES FOR PROTEIN-PURIFICATION

被引：22

作者：

CHEN, JP

机构：

[1] Center for Dairy Research, Department of Food Science, University of Wisconsin-Madison, Madison

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1990年 / 70卷 / 03期

关键词：

D O I：

10.1016/0922-338X(90)90188-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To circumvent the difficulties encountered in conventional affinity chromatography, novel affinity-based protein purification processes using reversible protein-ligand interactions have been developed. Depending on the physical means of isolating the ligand-ligate complex, the processes can be categorized as affinity precipitation, affinity cross-flow filtration, or affinity partition. This report reviews the current status of such purification techniques. © 1989.

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页码：199 / 209

页数：11

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