SPECIFIC TRANSCRIPTION OF CLONED METHANOBACTERIUM-THERMOAUTOTROPHICUM TRANSCRIPTION UNITS BY HOMOLOGOUS RNA-POLYMERASE INVITRO

被引：12

作者：

KNAUB, S

KLEIN, A

机构：

[1] Molecular Genetics, Department of Biology, Philipps University, D-3550 Marburg

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 06期

关键词：

D O I：

10.1093/nar/18.6.1441

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Specific in vitro transcription by partially purified RNA polymerase from Methanobacterium thermoautotrophicum of DNA sequences cloned in between the promoter and terminator regions of the methyl CoM reductase transcription unit of the same organism is described. The 5′-terminus of the product has been mapped. Deletion analyses of the promoter region show the limits of the sequences essential for the promoter function. © 1990 Oxford University Press.

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页码：1441 / 1446

页数：6

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