2 FORMS OF ELONGATION FACTOR-1-ALPHA (EF-1-ALPHA O AND 42SP50), PRESENT IN OOCYTES, BUT ABSENT IN SOMATIC-CELLS OF XENOPUS-LAEVIS

被引：20

作者：

DESCHAMPS, S

MORALES, J

MAZABRAUD, A

LEMAIRE, M

DENIS, H

BROWN, DD

机构：

[1] UNIV PARIS 06,INRA,PHYSIOL REPROD LAB,CNRS,UA 555,F-75252 PARIS 05,FRANCE

[2] CARNEGIE INST WASHINGTON,DEPT EMBRYOL,BALTIMORE,MD 21210

来源：

JOURNAL OF CELL BIOLOGY | 1991年 / 114卷 / 06期

关键词：

D O I：

10.1083/jcb.114.6.1109

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have purified and partially sequenced the EF-1-alpha protein from Xenopus laevis oocytes (EF-1-alpha-O). We show that the two cDNA clones isolated by Coppard et al. (Coppard, N. J., K. Poulsen, H. O. Madsen, J. Frydenberg, and B. F. C. Clark. 1991. J. Cell Biol. 112:237-243) do not encode 42Sp50, as claimed by these authors, but two very similar forms of EF-1-alpha-O (EF-1-alpha-O and EF-1-alpha-O1). 42Sp50 is the major protein component of a 42S nucleoprotein particle that is very abundant in previtellogenic oocytes of X. laevis. 42Sp50 differs from EF-1-alpha-O not only by its amino acid sequence, but also by several properties already reported. In particular, 42Sp50 has a low EF-1-alpha activity. It is distributed uniformly in the cytoplasm of previtellogenic oocytes, in contrast to EF-1-alpha-O which is concentrated in a small region of the cytoplasm, known as the mitochondrial mass or Balbiani body.

引用

页码：1109 / 1111

页数：3

共 14 条

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POTTING A, 1990, DIFFERENTIATION, V44, P103

[12] THESAURIN A, THE MAJOR PROTEIN OF XENOPUS-LAEVIS PREVITELLOGENIC OOCYTES, PRESENT IN THE 42 S PARTICLES, IS HOMOLOGOUS TO ELONGATION-FACTOR EF-1-ALPHA [J].