INHIBITION OF PNEUMOCYSTIS-CARINII DIHYDROPTEROATE SYNTHETASE BY SULFA DRUGS

被引：17

作者：

MERALI, S ^{[1
]}

ZHANG, Y ^{[1
]}

SLOAN, D ^{[1
]}

MESHNICK, S ^{[1
]}

机构：

[1] CUNY CITY COLL,DEPT MICROBIOL,NEW YORK,NY 10031

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1990年 / 34卷 / 06期

关键词：

D O I：

10.1128/AAC.34.6.1075

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new reversed-phase high-pressure liquid chromatography assay procedure for dihydropteroate synthetase (DHPS) that involves the elution of the enzyme incubation solution with a series of three solvents of decreasing polarity (ammonium phosphate buffer, 10% methanol, and 50% methanol) was designed. By this procedure DHPS was detected in Escherichia coli and Pneumocystis carinii with specific activities of 450 and 40 U/mg, respectively. A comparison of the effects of five sulfa drugs on P. carinii DHPS activity revealed that dapsone is the most potent of these drugs.

引用

页码：1075 / 1078

页数：4

共 16 条

[11] DAPSONE, TRIMETHOPRIM, AND SULFAMETHOXAZOLE PLASMA-LEVELS DURING TREATMENT OF PNEUMOCYSTIS PNEUMONIA IN PATIENTS WITH THE ACQUIRED IMMUNODEFICIENCY SYNDROME (AIDS) - EVIDENCE OF DRUG-INTERACTIONS
LEE, BL
MEDINA, I
BENOWITZ, NL
JACOB, P
WOFSY, CB
MILLS, J
[J]. ANNALS OF INTERNAL MEDICINE, 1989, 110 (08) : 606 - 611
[12] INHIBITION OF DIHYDROPTEROATE SYNTHETASE FROM ESCHERICHIA-COLI BY SULFONES AND SULFONAMIDES
MCCULLOUGH, JL
MAREN, TH
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1973, 3 (06) : 665 - 669
[13] DAPSONE TREATMENT OF PNEUMOCYSTIS-CARINII PNEUMONIA IN THE ACQUIRED IMMUNODEFICIENCY SYNDROME
MILLS, J
LEOUNG, G
MEDINA, I
HOPEWELL, PC
HUGHES, WT
WOFSY, C
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1988, 32 (07) : 1057 - 1060
[14] SEGAL IH, 1976, BIOCH CALCULATIONS, P246
[15] SHIOTA T, 1964, J BIOL CHEM, V239, P2259
[16] SHIOTA T, 1984, FOLATES PTERINS, V1, P121

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