HPLC ANALYSIS OF PHOSPHOLIPIDS BY EVAPORATIVE LASER LIGHT-SCATTERING DETECTION

被引：52

作者：

MOUNTS, TL

ABIDI, SL

RENNICK, KA

机构：

[1] Food Quality and Safety Research, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, 61604, Illinois

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1992年 / 69卷 / 05期

关键词：

CAPILLARY GAS CHROMATOGRAPHY; CRUDE SOYBEAN OIL; DAMAGED SOYBEANS; DEGUMMED SOYBEAN OIL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PHOSPHOLIPIDS;

D O I：

10.1007/BF02540944

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

High-performance liquid chromatography (HPLC for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soybeans subjected to storage at high moisture content. Analytical and preparative separations of phospholipids by normal-phase HPLC were accomplished. Major class fractions were analyzed by transmethylation and capillary column chromatography for fatty acid composition, and by reverse-phase C-18 HPLC (RP-HPLC) for molecular species composition. The RP-HPLC-ELSD system was limited to the analysis of phosphatidylcholine and phosphatidylethanolamine.

引用

页码：438 / 442

页数：5

共 26 条

[21]

NORMAN HA, 1986, J LIPID RES, V27, P1104

[22]

PATTON GM, 1982, J LIPID RES, V23, P190

[23]

ROBERTSO.JA, 1973, J AM OIL CHEM SOC, V50, P443, DOI 10.1007/BF02639850

[24] SEPARATION OF MAJOR PHOSPHOLIPID CLASSES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND SUBSEQUENT ANALYSIS OF PHOSPHOLIPID-BOUND FATTY-ACIDS USING GAS-CHROMATOGRAPHY [J].

SEEWALD, M ;

EICHINGER, HM .

JOURNAL OF CHROMATOGRAPHY, 1989, 469 :271-280

[25] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF SOYBEAN PHOSPHOLIPIDS [J].

SOTIRHOS, N ;

HO, CT ;

CHANG, SS .

FETTE SEIFEN ANSTRICHMITTEL, 1986, 88 (01) :6-8

[26] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PHOSPHOLIPIDS WITH UV DETECTION - OPTIMIZATION OF SEPARATIONS ON SILICA [J].

YANDRASITZ, JR ;

BERRY, G ;

SEGAL, S .

JOURNAL OF CHROMATOGRAPHY, 1981, 225 (02) :319-328

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