A PHYSICAL MAP OF THE GENOME OF HAEMOPHILUS-INFLUENZAE TYPE-B

被引：41

作者：

BUTLER, PD

MOXON, ER

机构：

[1] Department of Paediatrics, Institute of Mol. Medicine, University of Oxford

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷

关键词：

D O I：

10.1099/00221287-136-12-2333

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Contour-clamped homogeneous electric field pulsed-filed gel electrophoresis (PGFE) was used in combination with Southern hybridization to construct a genomic restriction map for the pathogen Haemophilus influenzae serotype b, strain Eagan. Sites for four restriction endonucleases, EagI, NaeI, RsrII and SmaI, were located on the 2100 kbp circular chromosome. Twelve potential virulence loci have been placed on the map together with certain loci essential for growth of the bacteria (e.g. ribosomal RNA operons). PFGE also provided a valuable tool for characterizing ten capsulated, type b isolates (other than Eagan) known to be genetically heterogeneous and two laboratory-derived variants (transformants) derived through complex recombinational events involving random uptake of high-molecular-mass donor genomic DNA.

引用

页码：2333 / 2342

页数：10

共 43 条

[11]

FEINBERG AP, 1984, ANAL BIOCHEM, V137, P266

[12] HAEMOPHILUS-INFLUENZAE IMMUNOGLOBULIN A1 PROTEASE GENES - CLONING BY PLASMID INTEGRATION-EXCISION, COMPARATIVE ANALYSES, AND LOCALIZATION OF SECRETION DETERMINANTS [J].