PESTICIDE-SENSITIVE ISFET BASED ON ENZYME-INHIBITION

被引：66

作者：

DUMSCHAT, C ^{[1
]}

MULLER, H ^{[1
]}

STEIN, K ^{[1
]}

SCHWEDT, G ^{[1
]}

机构：

[1] TECH UNIV CLAUSTHAL,INST ANORGAN & ANALYT CHEM,W-3392 CLAUSTHAL ZELLERFE,GERMANY

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 252卷 / 1-2期

关键词：

POTENTIOMETRY; BIOSENSORS; ENZYME INHIBITION; ION-SENSITIVE FIELD EFFECT TRANSISTORS; PESTICIDES; WATER;

D O I：

10.1016/0003-2670(91)87189-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple method for an enzyme inhibition test is described. The decrease in enzyme activity after incubation in a solution of dichlorvos as inhibitor is detected potentiometrically. The enzyme acetylcholinesterase is immobilized onto the pH-sensitive gate area of an ISFET. Such a biosensor is able to detect dichlorvos in water in the concentration range from 0.3 to 1.2 mg l-1 without preconcentration.

引用

页码：7 / 9

页数：3

共 12 条

[1] PENICILLIN-G SENSOR BASED ON PENICILLIN AMIDASE COUPLED TO A FIELD-EFFECT TRANSISTOR [J].

BRAND, U ;

SCHEPER, T ;

SCHUGERL, K .

ANALYTICA CHIMICA ACTA, 1989, 226 (01) :87-97

[2] ENCAPSULATION OF CHEMICALLY SENSITIVE FIELD-EFFECT TRANSISTORS WITH PHOTOCURABLE EPOXY-RESINS [J].

DUMSCHAT, C ;

MULLER, H ;

RAUTSCHEK, H ;

TIMPE, HJ ;

HOFFMANN, W ;

PHAM, MT ;

HULLER, J .

SENSORS AND ACTUATORS B-CHEMICAL, 1990, 2 (04) :271-276

[3] DETECTION OF ORGANOPHOSPHOROUS PESTICIDES WITH AN IMMOBILIZED CHOLINESTERASE ELECTRODE [J].

DURAND, P ;

NICAUD, JM ;

MALLEVIALLE, J .

JOURNAL OF ANALYTICAL TOXICOLOGY, 1984, 8 (03) :112-117

[4] MICRO-ENZYME FIELD-EFFECT TRANSISTOR SENSOR USING DIRECT COVALENT BONDING OF UREASE [J].

GARDIES, F ;

JAFFREZICRENAULT, N ;

MARTELET, C ;

PERROT, H ;

VALLETON, JM ;

ALEGRET, S .

ANALYTICA CHIMICA ACTA, 1990, 231 (02) :305-308

[5]

Goodson L H, 1976, Methods Enzymol, V44, P647

[6] GLUCOSE SENSOR BASED ON A FIELD-EFFECT TRANSISTOR WITH A PHOTOLITHOGRAPHICALLY PATTERNED GLUCOSE-OXIDASE MEMBRANE [J].

HANAZATO, Y ;

NAKAKO, M ;

MAEDA, M ;

SHIONO, S .

ANALYTICA CHIMICA ACTA, 1987, 193 :87-96

[7]

HERZSPRUNG L, 1990, WASSER, V74, P339

[8]

HINTSCHE R, 1989, ANAL LETT, V22, P2175

[9] EXCHANGEABLE IMMOBILIZED ENZYME REACTOR FOR ENZYME-INHIBITION TESTS IN FLOW-INJECTION ANALYSIS USING A MAGNETIC DEVICE - DETERMINATION OF PESTICIDES IN DRINKING-WATER [J].

KINDERVATER, R ;

KUNNECKE, W ;

SCHMID, RD .

ANALYTICA CHIMICA ACTA, 1990, 234 (01) :113-117

[10] FLOW-INJECTION DETERMINATION OF PARAOXON BY INHIBITION OF IMMOBILIZED ACETYLCHOLINESTERASE [J].

LEONGONZALEZ, ME ;

TOWNSHEND, A .

ANALYTICA CHIMICA ACTA, 1990, 236 (02) :267-272

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