OXIDATION OF LOW-DENSITY LIPOPROTEINS BY OH. AND OH./02-RADICAL-ANION FREE-RADICALS PRODUCED BY GAMMA-RADIOLYSIS

被引：42

作者：

BONNEFONTROUSSELOT, D ^{[1
]}

GARDESALBERT, M ^{[1
]}

DELATTRE, J ^{[1
]}

FERRADINI, C ^{[1
]}

机构：

[1] UNIV PARIS 05, CHIM PHYS LAB, F-75270 PARIS 06, FRANCE

来源：

RADIATION RESEARCH | 1993年 / 134卷 / 03期

关键词：

D O I：

10.2307/3578186

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this study was to analyze quantitatively the oxidative modification of low-density lipoproteins (LDLs) induced by OH· free radicals produced by gamma radiolysis, in the absence or in the presence of oxygen (action of OH· free radicals, or simultaneous action of OH· and O2·- free radicals, respectively). The effects of increasing radiation doses on aqueous LDL solutions have been monitored by several parameters: a decrease in endogenous vitamin E, the formation of thiobarbituric acid-reactive substances (TBARS) and conjugated dienes, the appearance of a differential fluorescence (excitation wavelength = 360 nm), and an increase in the relative electrophoretic mobility. Initial radiation yields (decrease in vitamin E, formation of TBARS) have been determined at pH 7 as a function of LDL concentration (from 0.75 to 9 g liter-1). From the comparison of these yields with those of OH· and O2·- free radicals produced by water radiolysis, we have deduced reaction mechanisms for the initiation of oxidation of LDLs by OH· and OH·/O2·- free radicals.

引用

页码：271 / 282

页数：12

共 28 条

[11] LOW-DENSITY LIPOPROTEIN PATHWAY AND ITS RELATION TO ATHEROSCLEROSIS [J].

GOLDSTEIN, JL ;

BROWN, MS .

ANNUAL REVIEW OF BIOCHEMISTRY, 1977, 46 :897-930

[12]

HABERLAND ME, 1984, J BIOL CHEM, V259, P1305

[13]

HARTMANN V, 1970, Z NATURFORSCH PT B, VB 25, P1394

[14] DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUM [J].

HAVEL, RJ ;

EDER, HA ;

BRAGDON, JH .

JOURNAL OF CLINICAL INVESTIGATION, 1955, 34 (09) :1345-1353

[15] IRON AND COPPER PROMOTE MODIFICATION OF LOW-DENSITY LIPOPROTEIN BY HUMAN ARTERIAL SMOOTH-MUSCLE CELLS IN CULTURE [J].

HEINECKE, JW ;

ROSEN, H ;

CHAIT, A .

JOURNAL OF CLINICAL INVESTIGATION, 1984, 74 (05) :1890-1894

[16] FREE-RADICAL MODIFICATION OF LOW-DENSITY-LIPOPROTEIN - MECHANISMS AND BIOLOGICAL CONSEQUENCES [J].

HEINECKE, JW .

FREE RADICAL BIOLOGY AND MEDICINE, 1987, 3 (01) :65-73

[17] SUPEROXIDE-MEDIATED MODIFICATION OF LOW-DENSITY-LIPOPROTEIN BY ARTERIAL SMOOTH-MUSCLE CELLS [J].

HEINECKE, JW ;

BAKER, L ;

ROSEN, H ;

CHAIT, A .

JOURNAL OF CLINICAL INVESTIGATION, 1986, 77 (03) :757-761

[18]

JORE D, 1986, RADIAT PHYS CHEM, V28, P557

[19]

LANGFINGER D, 1985, Z NATURFORSCH C, V40, P446

[20] PACKING OF CHOLESTEROL MOLECULES IN HUMAN LOW-DENSITY-LIPOPROTEIN [J].

LUNDKATZ, S ;

PHILLIPS, MC .

BIOCHEMISTRY, 1986, 25 (07) :1562-1568

← 1 2 3 →