ASSESSMENT OF LEWIS BLOOD-GROUP ANTIGENS AND SECRETOR STATUS IN AUTOPSY SPECIMENS

被引：1

作者：

BUSUTTIL, A

BLACKWELL, CC

JAMES, VS

MACKENZIE, DAC

SAADI, AT

WEIR, DM

机构：

[1] UNIV EDINBURGH,DEPT FORENS MED,EDINBURGH EH8 9AG,MIDLOTHIAN,SCOTLAND

[2] UNIV EDINBURGH,SCH MED,DEPT MED MICROBIOL,TEVIOT PL,EDINBURGH EH8 9AG,MIDLOTHIAN,SCOTLAND

来源：

FORENSIC SCIENCE INTERNATIONAL | 1993年 / 61卷 / 2-3期

关键词：

SECRETOR STATUS; LEWIS BLOOD GROUP; AUTOPSY SPECIMENS;

D O I：

10.1016/0379-0738(93)90221-U

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

The ability of enzyme linked immunosorbent assays (ELISA) to detect Lewis and H antigens in secretions obtained from 280 autopsies was assessed. The ELISA results were comPared with those for matched blood specimens examined by agglutination of erythrocytes by monoclonal anti-Lewis(a) and anti-Lewis(b) antibodies. There was good agreement between the results for the two tests and the ELISAs could be used to determine secretor status of the subject. While determination of ABO group with monoclonal anti-A and anti-B was possible even with lysed blood, the results for Lewis typing by erythrocyte agglutination were poor if the sample was lysed or partially lysed. Detection of the antigens by ELISA was as efficient among elderly subjects as among younger ones and both H and Lewis antigens could be detected on erythrocytes and in secretions up to 127 h after death.

引用

页码：133 / 140

页数：8

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