SENSITIVE FLUORESCENCE DETECTION IN CAPILLARY ELECTROPHORESIS USING LASER-DIODES AND MULTIPLEX DYES

被引：19

作者：

BACHTELER, G

DREXHAGE, KH

ARDENJACOB, J

HAN, KT

KOLLNER, M

MULLER, R

SAUER, M

SEEGER, S

WOLFRUM, J

机构：

[1] UNIV HEIDELBERG,INST PHYS CHEM,NEUENHEIMER FELD 253,D-69120 HEIDELBERG,GERMANY

[2] UNIV GESAMTHSCH SIEGEN,INST PHYS CHEM,D-57068 SIEGEN,GERMANY

来源：

JOURNAL OF LUMINESCENCE | 1994年 / 62卷 / 3-4期

关键词：

D O I：

10.1016/0022-2313(94)90336-0

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A detector for laser induced fluorescence based on laser diode technology was designed and sensitivity measurements with rhodamine 800 were performed. The detection limit is around 100 molecules in the detection volume. Using the laser diode in combination with a commercial capillary electrophoresis system, about 1200 molecules were detectable. Using time-resolved fluorescence measurements multiplex dyes were recognized with a pulsed laser diode and a pattern recognition technique collecting only a few hundred photons.

引用

页码：101 / 108

页数：8

共 24 条

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[2]

Brackmann U, 1986, LAMBDACHROME LASER D

[3]

BRIGHT FV, 1988, ANAL CHEM, V60, pA3031

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