ISOLATION OF CDNAS ENCODING SUBUNIT VIB OF HUMAN CYTOCHROME-C-OXIDASE AND STEADY-STATE LEVELS OF COXVIB MESSENGER-RNA IN DIFFERENT TISSUES

被引:17
作者
TAANMAN, JW
SCHRAGE, C
PONNE, NJ
DAS, AT
BOLHUIS, PA
DEVRIES, H
AGSTERIBBE, E
机构
[1] UNIV AMSTERDAM,ACAD MED CTR,DEPT NEUROL,1105 AZ AMSTERDAM,NETHERLANDS
[2] UNIV AMSTERDAM,DEPT ANAT & EMBRYOL,1105 AZ AMSTERDAM,NETHERLANDS
关键词
antisera; fibroblasts; liver; Mitochondrial protein; MOLT-4; cells; nuclear gene; respiratory chain; skeletal muscle; λgt11 and λZAP libraries;
D O I
10.1016/0378-1119(90)90237-L
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A full-length cDNA clone specifying the nuclear-encoded subunit VIb of human cytochrome c oxidase (COX) was isolated from a human skeletal muscle cDNA expression library. This was done with antiserum directed against the group of subunits VIa, b and c of bovine heart COX. A potential ribosome-binding site was located immediately upstream from the initiation codon. The predicted amino acid sequence revealed 85% similarity with the corresponding subunit of bovine heart COX. Subunit VIb lacks a cleavable presequence for mitochondrial addressing. We assume that there are no tissue-specific isoforms of subunit VIb, since (i) in a Northern blot experiment a single hybridizing band of approx. 500 nucleotides was demonstrated in RNA from liver, skeletal muscle, MOLT-4 cells and fibroblasts and (ii) a full-length cDNA clone with an identical sequence was isolated from a human liver cDNA library. Steady-state levels of the coxVIb transcript were different in the tissues examined. © 1990.
引用
收藏
页码:285 / 291
页数:7
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