CHARACTERIZATION OF A GLUCOAMYLASE IMMOBILIZED ON CHITIN

被引：6

作者：

FREIRE, DG

SANTANNA, GL

机构：

[1] UNIV FED RIO DE JANEIRO,ESCOLA QUIM,COPPE,POB 68502,BR-21941 RIO DE JANEIRO,RJ,BRAZIL

[2] UNIV FED RIO DE JANEIRO,INST QUIM,BR-21941 RIO DE JANEIRO,RJ,BRAZIL

来源：

BIOMASS | 1990年 / 23卷 / 01期

关键词：

enzyme immobilization; glucoamylase; manioc starch hydrolysis;

D O I：

10.1016/0144-4565(90)90074-T

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Glucoamylase (E.C. 3.2.1.3) covalently immobilized on chitin particles (24-60 mesh) showed an average activity of 1000 U g-1. Temperature and pH optima were 60°C and 3·6, respectively. These values were lower than the corresponding ones for the free enzyme (pH 4·4, temperature 65°C). The Km (Michaelis constant) values for soluble starch and hydrolyzed manioc starch were, respectively, 1·25 and 3·94 g litre-1 (free enzyme) and 8·6 and 7·8 g litre-1 (immobilized enzyme). © 1990.

引用

页码：71 / 78

页数：8

共 20 条

[1] STARCH CONVERSION BY IMMOBILIZED GLUCOAMYLASE [J].