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USE OF MOLECULAR-CLONING METHODS TO MAP THE DISTRIBUTION OF EPITOPES ON TOPOISOMERASE-I (SCL-70) RECOGNIZED BY SERA OF SCLERODERMA PATIENTS

被引：46
作者：
DARPA, P
WHITECOOPER, H
CLEVELAND, DW
ROTHFIELD, NF
EARNSHAW, WC
机构：
[1] JOHNS HOPKINS UNIV, SCH MED, DEPT CELL BIOL & ANAT, 725 N WOLFE ST, BALTIMORE, MD 21205 USA
[2] JOHNS HOPKINS UNIV, SCH MED, DEPT BIOL CHEM, BALTIMORE, MD 21205 USA
[3] UNIV CONNECTICUT, CTR HLTH, DEPT MED, DIV RHEUMAT DIS, FARMINGTON, CT 06032 USA
来源：
ARTHRITIS AND RHEUMATISM | 1990年 / 33卷 / 10期
关键词：
D O I：
10.1002/art.1780331007
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
We report the initial molecular characterization of the autoimmune response against DNA topoisomerase I (topo I; Scl‐70). Sera from 36 patients with scleroderma and 4 healthy control subjects were studied using 6 subcloned portions of topo I. Twenty‐three sera recognized at least 2 independent epitopes on the molecule. Therefore, anti‐topo I, like other non‐organ‐specific autoantibodies characterized to date, is polyclonal and multifocal. The cloned protein should prove suitable for sensitive early detection of anti‐topo I in the clinical setting. Copyright © 1990 American College of Rheumatology
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页码：1501 / 1511
页数：11
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