ISOTOPE RATIOS OF MOLYBDENUM DETERMINED BY THERMAL IONIZATION MASS-SPECTROMETRY FOR STABLE-ISOTOPE STUDIES OF MOLYBDENUM METABOLISM IN HUMANS

被引：51

作者：

TURNLUND, JR

KEYES, WR

PEIFFER, GL

机构：

[1] USDA/ARS, Western Human Nutrition Research Center, California 94129, P.O. Box 29997, Presidio of San Francisco

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 13期

关键词：

D O I：

10.1021/ac00061a014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Methods were developed to separate and purify Mo from biological samples and to measure isotopic ratios in 1 mug of Mo. A magnetic sector, thermal ionization mass spectrometer was used with simultaneous collection of five isotopes. Isotopic ratios were corrected for mass fractionation by iterative normalization using the 96/98 ratio. Ion beam intensity was enhanced by using a double-filament configuration, loading samples onto evaporation filaments with silica gel and boric acid. A triple-isotope-dilution approach was used, so the method could be applied to two-tracer studies of Mo metabolism in human subjects. Mo-94 was added to samples prior to purification to quantify the total Mo content of samples and to determine the amounts of enriched Mo-97 and Mo-100 appearing in urine and fecal samples of study participants. The three ratios, 94/98, 97/98, and 100/98, were determined with within-run precision of from 0.06 to 0.10% (RSD). Precision of the ratios between replicates was from 0.05 to 0.08%.

引用

页码：1717 / 1722

页数：6

共 18 条

[1]

[Anonymous], 1989, RECOMMENDED DIETARY

[2] ISOTOPIC COMPOSITION + ATOMIC WEIGHT OF NATURALLY OCCURRING MOLYBDENUM - POSSIBLE REFLEXION OF CREATION PROCESS [J].

CROUCH, EAC ;

TUPLIN, TA .

NATURE, 1964, 202 (493) :1282-&

[3] TABLE OF THE ISOTOPIC COMPOSITION OF THE ELEMENTS AS DETERMINED BY MASS-SPECTROMETRY [J].

DEBIEVRE, P ;

BARNES, IL .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1985, 65 (1-2) :211-230

[4]

Dixon W.J., 1951, ANN MATH STAT, V22, P68, DOI [DOI 10.1214/AOMS/1177729693, 10.1214/aoms/1177729693]

[5] ISOTOPE-DILUTION MASS-SPECTROMETRY FOR ACCURATE ELEMENTAL ANALYSIS [J].

FASSETT, JD ;

PAULSEN, PJ .

ANALYTICAL CHEMISTRY, 1989, 61 (10) :A643-&

[6]

Heumann K.G., 1988, INORGANIC MASS SPECT, P301

[7] CONCENTRATION-GRADIENT ELUTION OF METAL CHLORIDE COMPLEXES FROM AN ANION-EXCHANGE RESIN [J].

HICKS, HG ;

STEVENSON, PC ;

SCHWEIGER, JS .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1978, 16 (11) :527-533

[8] INTERNAL NORMALIZATION TECHNIQUES FOR HIGH ACCURACY ISOTOPE-DILUTION ANALYSES - APPLICATION TO MOLYBDENUM AND NICKEL IN STANDARD REFERENCE MATERIALS [J].

MOORE, LJ ;

MACHLAN, LA ;

SHIELDS, WR ;

GARNER, EL .

ANALYTICAL CHEMISTRY, 1974, 46 (08) :1082-1089

[9]

MURTHY VR, 1963, GEOCHIM COSMOCHIM AC, V27, P1171

[10] HIGH-ACCURACY MEASUREMENT OF ISOTOPE RATIOS OF MOLYBDENUM IN SOME TERRESTRIAL MOLYBDENITES [J].

QILU ;

MASUDA, A .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1992, 3 (01) :10-17

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