BSR-REMI - AN IMPROVED METHOD FOR GENE TAGGING USING A NEW VECTOR IN DICTYOSTELIUM

被引：12

作者：

MORIO, T ^{[1
]}

ADACHI, H ^{[1
]}

SUTOH, K ^{[1
]}

YANAGISAWA, K ^{[1
]}

TANAKA, Y ^{[1
]}

机构：

[1] UNIV TOKYO,GRAD SCH ARTS & SCI,DEPT LIFE SCI,MEGURO KU,TOKYO 153,JAPAN

来源：

JOURNAL OF PLANT RESEARCH | 1995年 / 108卷 / 1089期

关键词：

BSR-REMI; DICTYOSTELIUM DISCOIDEUM; INSERTIONAL MUTAGENESIS;

D O I：

10.1007/BF02344314

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Using a plasmid pBsr2 which carries a blasticidin S-resistant gene, we have improved the method of REMI (restriction enzyme-mediated integration) provided for insertional mutagenesis in Dictyostelium discoideum (bsr-REMI). To confirm usefulness of the bsr-REMI, transformation efficiency, copy number of integrated DNA, and randomness of integration into genome were examined.

引用

页码：111 / 114

页数：4

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