3-HYDROXY-3-METHYLGLUTARYLDITHIO-COENZYME-A - A POTENT INHIBITOR OF PSEUDOMONAS-MEVALONII HMG-COA REDUCTASE

被引：8

作者：

WRENSFORD, LV ^{[1
]}

RODWELL, VW ^{[1
]}

ANDERSON, VE ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907

来源：

BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY | 1991年 / 45卷 / 02期

关键词：

D O I：

10.1016/0885-4505(91)90022-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3-Hydroxy-3-methyl-1-thionoglutaryl-coenzyme A, a dithioester analog of 3-hydroxy-3-methylglutaryl-CoA, has been enzymatically synthesized using the HMG-CoA synthase catalyzed condensation of acetyl-CoA with 3-oxo-1-thionobutyryl-CoA. HMGdithio-CoA is a potent inhibitor of Pseudomonas mevalonii HMG-CoA reductase. Inhibition was mainly competitive with respect to HMG-CoA with a Kis of 0.086 ± .01 μm and noncompetitive with respect to NADH with a Kis of 3.7 ± 1.5 μm and a Kii of 0.65 ± .05 μm in the presence of 110 μm (R,S)-HMG-CoA. © 1991.

引用

页码：204 / 208

页数：5

共 14 条

[1]

ANDERSON VE, 1990, J BIOL CHEM, V265, P6255

[2] CLONING, SEQUENCING, AND OVEREXPRESSION OF MVAA, WHICH ENCODES PSEUDOMONAS-MEVALONII 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE [J].