IDENTIFICATION AND INSITU DETECTION OF INDIVIDUAL BACTERIAL-CELLS

被引:14
作者
AMANN, R [1 ]
LUDWIG, W [1 ]
SCHLEIFER, KH [1 ]
机构
[1] TECH UNIV MUNICH,LEHRSTUHL MIKROBIOL,ARCISSTR 21,W-8000 MUNICH 2,GERMANY
关键词
INSITU IDENTIFICATION; OLIGONUCLEOTIDE PROBE; NONCULTURABLE BACTERIA;
D O I
10.1111/j.1574-6968.1992.tb05680.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Microbial ecology has long been hampered by the fact that most microorganisms cannot be identified in situ because of the lack of morphological diversity. The immunofluorescence approach has yielded important insights into the spatial distribution of microorganisms but has its severe limitations. The recently introduced fluorescently labelled, ribosomal RNA-targeted oligonucleotide probes have successfully been applied for the detection and identification in situ of individual microbial cells and evade some of the principal problems of fluorescent antibodies. The design and synthesis of these phylogenetically nested probes does not require cultivation and isolation of the target organism and can therefore be used to monitor the population distribution and dynamics of hitherto uncultured microorganisms.
引用
收藏
页码:45 / 50
页数:6
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