CHARACTERIZATION OF THE BSTV IRM GENES ENCODING THE BACILLUS-STEAROTHERMOPHILUS-V RESTRICTION-MODIFICATION SYSTEM

被引：6

作者：

GONZALEZ, E ^{[1
]}

VASQUEZ, C ^{[1
]}

机构：

[1] UNIV TALCA,DEPT CIENCIAS BIOL,BIOL MOLEC LAB,CASILLA 747,TALCA,CHILE

来源：

GENE | 1993年 / 131卷 / 01期

关键词：

RECOMBINANT DNA; HETEROLOGOUS GENE EXPRESSION; DNA SEQUENCING; PAER7I R-M SYSTEM; ENDONUCLEASE; METHYLTRANSFERASE;

D O I：

10.1016/0378-1119(93)90676-T

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide (nt) sequence of a 2.7-kb HindIII-EcoRI DNA fragment encoding the bstVIR and bstVIM genes has been determined. The sequence predicts a restriction endonuclease of 224 amino acids (aa), M(r) 25 104, and a methyl-transferase of 561 aa, M(r) 65 702. Both genes are aligned in the same orientation and are separated by a 102-nt intergenic region. No homology was found between R.BstVI and M.BstVI when their deduced aa sequences were compared. Significant similarity at the aa level was found, however, when both enzymes were compared to their equivalents in the paeR7IRM system of Pseudomonas aeruginosa PAO303.

引用

页码：103 / 106

页数：4

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