IMMOBILIZATION OF A FRUCTOSYL-TRANSFERRING ENZYME FROM AUREOBASIDIUM SP ON SHIRASU POROUS-GLASS

被引：30

作者：

HAYASHI, S

ITO, K

NONOGUCHI, M

TAKASAKI, Y

IMADA, K

机构：

[1] Department of Industrial Chemistry, Faculty of Engineering, Miyazaki University, Miyazaki, 889-21, 1-1, Gakuen Kibanadai Nishi

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1991年 / 72卷 / 01期

关键词：

D O I：

10.1016/0922-338X(91)90150-F

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A fructosyl-transferring enzyme from Aureobasidium sp. ATCC 20524 which produces 1-kestose from sucrose was immobilized onto a shirasu porous glass (1142 U g-1 support). The enzymatic profiles of the preparation were almost identical to those of the native one except that its stability was improved. The column packed with it was stable for more than 30 d during a continuous operation.

引用

页码：68 / 70

页数：3

共 11 条

[1] MONITORING OF REACTIONS IN SOLID-PHASE PEPTIDE SYNTHESIS WITH PICRIC ACID [J].

GISIN, BF .

ANALYTICA CHIMICA ACTA, 1972, 58 (01) :248-&

[2] PRODUCTION OF A FRUCTOSYL-TRANSFERRING ENZYME BY AUREOBASIDIUM SP ATCC 20524 [J].

HAYASHI, S ;

NONOKUCHI, M ;

IMADA, K ;

UENO, H .

JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1990, 5 (06) :395-400

[3] OBSERVATION OF THE CHEMICAL-STRUCTURE OF FRUCTOOLIGOSACCHARIDE PRODUCED BY AN ENZYME FROM AUREOBASIDIUM SP ATCC-20524 [J].

HAYASHI, S ;

IMADA, K ;

KUSHIMA, Y ;

UENO, H .

CURRENT MICROBIOLOGY, 1989, 19 (03) :175-177

[4]

HIDAKA H, 1987, NIPPON NOGEIK KAISHI, V61, P915

[5] FORMATION OF FRUCTOOLIGOSACCHARIDES FROM SUCROSE CATALYZED BY IMMOBILIZED BETA-FRUCTOFURANOSIDASE ORIGINATED FROM ASPERGILLUS-ORYZAE [J].

KIDA, M ;

YOSHIKAWA, T ;

SENDA, T ;

YOSHIHIRO, Y .

NIPPON KAGAKU KAISHI, 1988, (11) :1830-1835

[6] GLUCOAMYLASE COVALENTLY COUPLED TO POROUS-GLASS [J].

LI, GX ;

HUANG, JY ;

KOU, XF ;

ZHANG, SZ .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1982, 7 (05) :325-341

[7]

NAKASHIMA T, 1981, NIPPON KAGAKU KAISHI, P1231, DOI 10.1246/nikkashi.1981.1231

[8] IMMOBILIZATION OF BACILLUS-SUBTILIS ALPHA-AMYLASE ON ZIRCONIA-COATED ALKYLAMINE GLASS WITH GLUTARALDEHYDE [J].

RAMESH, V ;

SINGH, C .

ENZYME AND MICROBIAL TECHNOLOGY, 1981, 3 (03) :246-248

[9]

Weetall H H, 1976, Methods Enzymol, V44, P134

[10] ALKALINE PHOSPHATASE INSOLUBILIZED BY COVALENT LINKAGE TO POROUS GLASS [J].

WEETALL, HH .

NATURE, 1969, 223 (5209) :959-+

← 1 2 →