TRANSGENIC MICE CARRYING THE HUMAN POLIOVIRUS RECEPTOR - NEW ANIMAL-MODEL FOR STUDY OF POLIOVIRUS NEUROVIRULENCE

被引：87

作者：

HORIE, H

KOIKE, S

KURATA, T

SATOYOSHIDA, Y

ISE, I

OTA, Y

ABE, S

HIOKI, K

KATO, H

TAYA, C

NOMURA, T

HASHIZUME, S

YONEKAWA, H

NOMOTO, A

机构：

[1] UNIV TOKYO,INST MED SCI,DEPT MICROBIOL,MINATO KU,TOKYO,TOKYO 108,JAPAN

[2] TOKYO METROPOLITAN INST MED SCI,DEPT MICROBIOL,BUNKYO KU,TOKYO,TOKYO 113,JAPAN

[3] TOKYO METROPOLITAN INST MED SCI,DEPT LAB ANIM SCI,BUNKYO KU,TOKYO,TOKYO 113,JAPAN

[4] NATL INST HLTH,DEPT PATHOL,SHINJUKU KU,TOKYO,TOKYO 162,JAPAN

[5] JAPAN POLIOMYELITIS RES INST,TOKYO,TOKYO 189,JAPAN

[6] CENT INST EXPTL ANIM,MIYAMAE KU,KAWASAKI,KANAGAWA 216,JAPAN

来源：

JOURNAL OF VIROLOGY | 1994年 / 68卷 / 02期

关键词：

D O I：

10.1128/JVI.68.2.681-688.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recombinant viruses between the virulent Mahoney and attenuated Sabin 1 strains of poliovirus type 1 were subjected to neurovirulence tests using a transgenic (Tg) mouse line, ICR-PVRTg1, that carried the human poliovirus receptor gene. The Tg mice were inoculated intracerebrally with these recombinant viruses and observed for clinical signs, histopathological lesions, and viral antigens as parameters of neurovirulence of the viruses. These parameters observed in the Tg mice were different for different inoculated viruses. Dose-dependent incidences of paralysis and of death were observed in the Tg mice inoculated with any viruses used. This indicates that values of 50% lethal dose are useful to score a wide range of neurovirulence of poliovirus. The neurovirulence of individual viruses estimated by the Tg mouse model had a strong correlation with those estimated by monkey model. Consequently, the mouse tests identified the neurovirulence determinants on the genome of poliovirus that had been identified by monkey tests. In addition, the mouse tests revealed new neurovirulence determinants, that is, different nucleotides between the two strains at positions 189 and 21 and/or 935 in the 5'-proximal 1,122 nucleotides. The Tg mice used in this study may be suitable for replacing monkeys for investigating poliovirus neurovirulence.

引用

页码：681 / 688

页数：8

共 36 条

[31] NEW MODEL FOR THE SECONDARY STRUCTURE OF THE 5' NON-CODING RNA OF POLIOVIRUS IS SUPPORTED BY BIOCHEMICAL AND GENETIC DATA THAT ALSO SHOW THAT RNA SECONDARY STRUCTURE IS IMPORTANT IN NEUROVIRULENCE [J].