UPTAKE OF THIAMIN BY ISOLATED RAT-LIVER MITOCHONDRIA

被引：15

作者：

BARILE, M

PASSARELLA, S

QUAGLIARIELLO, E

机构：

[1] UNIV BARI, CNR, DIPARTIMENTO BIOCHIM & BIOL MOLEC, I-70126 BARI, ITALY

[2] UNIV BARI, CNR, CTR STUDIO MITOCONDRI & METAB ENERGET, I-70126 BARI, ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1986年 / 141卷 / 02期

关键词：

D O I：

10.1016/S0006-291X(86)80196-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Investigation is made here of 14C-thiamin uptake by rat liver mitochondria in vitro. Following incubation with mitochondria, 14C-thiamin remained in the mitochondrial pellet in spite of several washings of the organelles. Accordingly, externally added thiamin produced intra/extramitochondrial concentrations up to 5.4 and 14C-thiamin space/3H2O space ratios higher than one. These ratios decreased with increasing vitamin concentrations, thus suggesting the occurrence of saturation characteristics for vitamin uptake into mitochondria. Thiamin was proven to enter both intermembrane and matrix spaces, where neither binding to intramitochondrial protein nor phosphorylation were found to occur. Moreover thiamin uptake inhibition by both metal ions and certain thiamin analogues was also found.

引用

页码：466 / 473

页数：8

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