Preparation of Astragalus membranaceus lectin and evaluation of its biological function

被引：17

作者：

Bai, Chong-Zhi ^{[1
,2
]}

Hao, Jian-Qing ^{[3
]}

Hao, Xu-Liang ^{[1
]}

Feng, Ma-Li ^{[1
]}

机构：

[1] Chinese Med Hosp Shanxi Prov, Cent Lab, 16 Binghou Xijie, Taiyuan 030012, Shanxi, Peoples R China

[2] Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 010010, Peoples R China

[3] Shanxi Med Univ, Sch Basic Med Sci, Inst Biotechnol, Taiyuan 030012, Shanxi, Peoples R China

来源：

BIOMEDICAL REPORTS | 2018年 / 9卷 / 04期

关键词：

Astragalus membranaceus; lectin; coagulation activity; antitumor; antibacterial;

D O I：

10.3892/br.2018.1132

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

Astragalus membranaceus lectin (AML) was abstracted as a supposedly novel agglutinin of 67 kDa from the seeds of Astragalus membranaceus. The seeds of Astragalus membranaceus were treated with acetate, ammonium sulfate precipitation, and purified by HiTrap SP XL ion column and Superdex G25 gel filtration chromatography to obtain the AML. AML contained 16.4% sugar, similar to 70% polar amino acids and similar to 30% hydrophobic amino acids. The AML exhibited agglutination activity toward human and animal erythrocytes, particularly human blood type O and rabbit erythrocytes. It also exhibited acid/alkali resistance and thermal denaturation above 64 degrees C. Compared with human normal liver HL-7702 cells, different concentrations of AML (6.25, 12.50, 25.00 and 50.00 mu g/ml) exhibited superior inhibitory effects on the growth of SGC-7901, HepG2 and H22 carcinoma cell lines, and displayed marked antibacterial effects on bacteria; the half maximal inhibitory concentration for B. dysenteriae, S. aureus and E. coli were 85.4, 80.2 and 65.3 mu g/ml, respectively.

引用

页码：345 / 349

页数：5

共 21 条

[1]

THE MANNOSE-SPECIFIC PLANT-LECTINS FROM CYMBIDIUM HYBRID AND EPIPACTIS-HELLEBORINE AND THE (N-ACETYLGLUCOSAMINE)N-SPECIFIC PLANT LECTIN FROM URTICA-DIOICA ARE POTENT AND SELECTIVE INHIBITORS OF HUMAN-IMMUNODEFICIENCY-VIRUS AND CYTOMEGALOVIRUS REPLICATION INVITRO [J].