FVB/N - AN INBRED MOUSE STRAIN PREFERABLE FOR TRANSGENIC ANALYSES

被引:509
作者
TAKETO, M
SCHROEDER, AC
MOBRAATEN, LE
GUNNING, KB
HANTEN, G
FOX, RR
RODERICK, TH
STEWART, CL
LILLY, F
HANSEN, CT
OVERBEEK, PA
机构
[1] BAYLOR UNIV, DEPT CELL BIOL, HOWARD HUGHES MED INST, HOUSTON, TX 77030 USA
[2] JACKSON LAB, BAR HARBOR, ME 04609 USA
[3] ROCHE INST MOLEC BIOL, DEPT CELL & DEV BIOL, NUTLEY, NJ 07110 USA
[4] YESHIVA UNIV ALBERT EINSTEIN COLL MED, DEPT MOLEC GENET, BRONX, NY 10461 USA
[5] NCI, BETHESDA, MD 20892 USA
关键词
ES CELLS; BLASTOCYST CHIMERA; GERM-LINE TRANSMISSION;
D O I
10.1073/pnas.88.6.2065
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
FVB/N mice offer a system suitable for most transgenic experiments and subsequent genetic analyses. The inbred FVB/N strain is characterized by vigorous reproductive performance and consistently large litters. Moreover, fertilized FVB/N eggs contain large and prominent pronuclei, which facilitate microinjection of DNA. The phenotype of large pronuclei in the zygote is a dominant trait associated with the FVB/N oocyte but not the FVB/N sperm. In experiments to generate transgenic mice, the same DNA constructs were injected into three different types of zygotes: FVB/N, C57BL/6J, and (C57BL/6J X SJL/J)F1. FVB/N zygotes survived well after injection, and transgenic animals were obtained with efficiencies similar to the F1 zygotes and much better than the C57BL/6J zygotes. Genetic markers of the FVB/N strain have been analyzed for 44 loci that cover 15 chromosomes and were compared with those of commonly used inbred strains. In addition to the albino FVB/N strain, pigmented congenic strains of FVB/N are being constructed. These features make the FVB/N strain advantageous to use for research with transgenic mice.
引用
收藏
页码:2065 / 2069
页数:5
相关论文
共 26 条
[21]   THE DEVELOPMENTAL CAPACITY OF MOUSE OOCYTES THAT MATURED SPONTANEOUSLY INVITRO IS NORMAL [J].
SCHROEDER, AC ;
EPPIG, JJ .
DEVELOPMENTAL BIOLOGY, 1984, 102 (02) :493-497
[22]   APPLICATION OF A PROTEIN-BLOTTING PROCEDURE TO THE STUDY OF HUMAN GLUCOCORTICOID RECEPTOR INTERACTIONS WITH DNA [J].
SILVA, CM ;
TULLY, DB ;
PETCH, LA ;
JEWELL, CM ;
CIDLOWSKI, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (07) :1744-1748
[23]   DNA EXTRACTION WITH ORGANIC-SOLVENTS IN GEL BARRIER TUBES [J].
THOMAS, SM ;
MORENO, RF ;
TILZER, LL .
NUCLEIC ACIDS RESEARCH, 1989, 17 (13) :5411-5411
[24]   GENE-TRANSFER INTO MURINE STEM-CELLS AND MICE USING RETROVIRAL VECTORS [J].
WAGNER, EF ;
KELLER, G ;
GILBOA, E ;
RUTHER, U ;
STEWART, C .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1985, 50 :691-700
[25]   AN INHERITED LIMB DEFORMITY CREATED BY INSERTIONAL MUTAGENESIS IN A TRANSGENIC MOUSE [J].
WOYCHIK, RP ;
STEWART, TA ;
DAVIS, LG ;
DEUSTACHIO, P ;
LEDER, P .
NATURE, 1985, 318 (6041) :36-40
[26]   CONSERVED CYSTEINE TO SERINE MUTATION IN TYROSINASE IS RESPONSIBLE FOR THE CLASSICAL ALBINO MUTATION IN LABORATORY MICE [J].
YOKOYAMA, T ;
SILVERSIDES, DW ;
WAYMIRE, KG ;
KWON, BS ;
TAKEUCHI, T ;
OVERBEEK, PA .
NUCLEIC ACIDS RESEARCH, 1990, 18 (24) :7293-7298