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FVB/N - AN INBRED MOUSE STRAIN PREFERABLE FOR TRANSGENIC ANALYSES
被引:509
作者:
TAKETO, M
SCHROEDER, AC
MOBRAATEN, LE
GUNNING, KB
HANTEN, G
FOX, RR
RODERICK, TH
STEWART, CL
LILLY, F
HANSEN, CT
OVERBEEK, PA
机构:
[1] BAYLOR UNIV, DEPT CELL BIOL, HOWARD HUGHES MED INST, HOUSTON, TX 77030 USA
[2] JACKSON LAB, BAR HARBOR, ME 04609 USA
[3] ROCHE INST MOLEC BIOL, DEPT CELL & DEV BIOL, NUTLEY, NJ 07110 USA
[4] YESHIVA UNIV ALBERT EINSTEIN COLL MED, DEPT MOLEC GENET, BRONX, NY 10461 USA
[5] NCI, BETHESDA, MD 20892 USA
来源:
关键词:
ES CELLS;
BLASTOCYST CHIMERA;
GERM-LINE TRANSMISSION;
D O I:
10.1073/pnas.88.6.2065
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
FVB/N mice offer a system suitable for most transgenic experiments and subsequent genetic analyses. The inbred FVB/N strain is characterized by vigorous reproductive performance and consistently large litters. Moreover, fertilized FVB/N eggs contain large and prominent pronuclei, which facilitate microinjection of DNA. The phenotype of large pronuclei in the zygote is a dominant trait associated with the FVB/N oocyte but not the FVB/N sperm. In experiments to generate transgenic mice, the same DNA constructs were injected into three different types of zygotes: FVB/N, C57BL/6J, and (C57BL/6J X SJL/J)F1. FVB/N zygotes survived well after injection, and transgenic animals were obtained with efficiencies similar to the F1 zygotes and much better than the C57BL/6J zygotes. Genetic markers of the FVB/N strain have been analyzed for 44 loci that cover 15 chromosomes and were compared with those of commonly used inbred strains. In addition to the albino FVB/N strain, pigmented congenic strains of FVB/N are being constructed. These features make the FVB/N strain advantageous to use for research with transgenic mice.
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页码:2065 / 2069
页数:5
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