INCORPORATION OF GLUCOSAMINE-14C INTO MEMBRANE PROTEINS OF RETICULOCYTES

被引：13

作者：

HARRIS, ED

JOHNSON, CA

机构：

[1] Department of Biological Chemistry, University of Illinois, College of Medicine, Chicago

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 02期

关键词：

D O I：

10.1021/bi00830a009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Reticulocytes from phenylhydrazine-treated rabbits were found to incorporate radioactive glucosamine into glycoproteins of the cell membrane. Hexosamines accounted for 90% of the bound activity, while less than 5 % was found in sialic acid. Radioactive uridine diphosphate A-acetylglucosamine was identified in the acid-soluble fraction. The incorporation was inhibited by puromycin. Added glucose was also inhibitory. Labeled components were cleaved from the intact cell surface by trypsin. These components showed a number of radioactive peaks on DEAE-cellulose and were found to be associated with glycopeptides of high molecular weight. About 10% of the radioactivity was extractable from stroma by organic solvents. © 1969, American Chemical Society. All rights reserved.

引用

页码：512 / +

页数：1

共 29 条

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