RESPONSE OF RED-CELL AND PLASMA-VOLUME TO PROLONGED TRAINING IN HUMANS

To clarify the role of progressive heavy training on vascular volumes and hematologic status, seven untrained males [maximal O2 uptake (VO2max) = 45.1 +/- 1.1 (SE) ml.kg-1.min-1] cycled 2 h/day at an estimated 62% of VO2max. Training was conducted five to six times per week for approximately 8 wk. During this time, VO2max increased (P < 0.05) by 17.2%. Plasma volume (PV) measured by I-125 increased (P < 0.05) from 3,068 +/- 104 ml at 0 wk to 3,490 +/- 126 ml at 4 wk and then plateaued during the remaining four wk (3,362 +/- 113 ml). Red cell (RBC) mass (RCM) measured by Cr-51-labeled RBC did not change during the initial 4 wk of training (2,247 +/- 66 vs. 2,309 +/- 128 ml). As well, no apparent change occurred in RCM during the final 4 wk of training when RCM was estimated using PV and hematocrit (Hct). Collectively, PV plus RCM, expressed as total blood volume (TBV), increased (P < 0.05) by 10% at 4 wk and then stabilized for the final 4 wk. During the initial phase of training, reductions (P < 0.05) were also noted in Hct (4.6%), hemoglobin (Hb, 4.0%), and RBC count (6.3%). In contrast, an increase in mean cell volume (MCV, 1.7%) and mean cell Hb (2.3%) was observed (P < 0.05). From 4 to 8 wk, no further changes (P > 0.05) in Hb, RBC, and MCV were found, whereas both mean cell Hb and Hct returned to pretraining levels. During the training, serum ferritin was reduced (P < 0.05) from 78.6 +/- 1.3 to 33.8 +/- 6.7-mu-g/l. These results indicate that the initial response to training is a hypervolemia resulting in a pseudoanemia. With continued training, the pseudoanemia persists. The increase in MCV with training suggests a hemolysis of older RBCs and replacement by younger erythrocytes.