PROPERTIES OF THE TUNGSTEN-SUBSTITUTED MOLYBDENUM FORMYLMETHANOFURAN DEHYDROGENASE FROM METHANOBACTERIUM-WOLFEI

被引：43

作者：

SCHMITZ, RA

ALBRACHT, SPJ

THAUER, RK

机构：

[1] MAX PLANCK INST TERRESTR MIKROBIOL,MARBURG,GERMANY

[2] UNIV AMSTERDAM,EC SLATER INST BIOCHEM RES,1018 NL AMSTERDAM,NETHERLANDS

来源：

FEBS LETTERS | 1992年 / 309卷 / 01期

关键词：

FORMYLMETHANOFURAN DEHYDROGENASE; TUNGSTEN-SUBSTITUTED MOLYBDENUM PROTEIN; TUNGSTEN EPR SIGNAL; METHANOGENIC ARCHAEA;

D O I：

10.1016/0014-5793(92)80743-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Methanobacterium wolfei two formylmethanofuran dehydrogenases are present. one of which is a molybdenum- and the other a tungsten enzyme. We report here that also the 'molybdenum' enzyme contained tungsten when the archaeon was grown on molybdenum-deprived medium supplemented with tungstate (1-mu-M). Unexpectedly the tungsten-substituted molybdenum enzyme was catalytically active and displayed a rhombic EPR signal which was attributed to tungsten by the characteristic W-183 splitting.

引用

页码：78 / 81

页数：4

共 24 条

[21] WEAST RC, 1978, CRC HDB CHEM PHYSICS
[22] CARBOXYLIC-ACID REDUCTASE - A NEW TUNGSTEN ENZYME CATALYZES THE REDUCTION OF NON-ACTIVATED CARBOXYLIC-ACIDS TO ALDEHYDES
WHITE, H
STROBL, G
FEICHT, R
SIMON, H
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 184 (01): : 89 - 96
[23] PURIFICATION AND SOME PROPERTIES OF THE TUNGSTEN-CONTAINING CARBOXYLIC-ACID REDUCTASE FROM CLOSTRIDIUM-FORMICOACETICUM
WHITE, H
FEICHT, R
HUBER, C
LOTTSPEICH, F
SIMON, H
[J]. BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1991, 372 (11): : 999 - 1005
[24] YAMAMOTO I, 1983, J BIOL CHEM, V258, P1826

← 1 2 3 →