G-WIRES - SELF-ASSEMBLY OF A TELOMERIC OLIGONUCLEOTIDE, D(GGGGTTGGGG), INTO LARGE SUPERSTRUCTURES

被引：147

作者：

MARSH, TC ^{[1
]}

HENDERSON, E ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL, DEPT ZOOL & GENET, AMES, IA 50011 USA

来源：

BIOCHEMISTRY | 1994年 / 33卷 / 35期

关键词：

D O I：

10.1021/bi00201a020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The telomeric DNA oligonucleotide 5'-G(4)T(2)G(4)-3' (Tet 1.5) spontaneously assembles into large superstructures we have termed G-wires. G-wires can be resolved by gel electrophoresis as a ladder pattern. The self-association of Tet 1.5 is noncovalent and exhibits characteristics of G4-DNA, a parallel four-stranded structure stabilized by guanine tetrads. Formation of G-wires is dependent upon the presence of Na+ and/or K+, and once formed, G-wires are resistant to denaturation. The results described here extend our understanding of the structural potential of G-rich nucleic acids and may provide insight into the possible roles of G-rich sequences and the novel structures they can form in biological systems.

引用

页码：10718 / 10724

页数：7

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