LIPOZYME DEACTIVATION BY BUTANOL AND TEMPERATURE

被引：15

作者：

GANDHI, NN ^{[1
]}

SAWANT, SB ^{[1
]}

JOSHI, JB ^{[1
]}

MUKESH, D ^{[1
]}

机构：

[1] ALCHEM RES CTR,THANA,MAHARASHTRA,INDIA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1995年 / 17卷 / 04期

关键词：

LIPOZYME; DEACTIVATION; BUTANOL; TEMPERATURE; SERIES-TYPE MECHANISM;

D O I：

10.1016/0141-0229(94)00073-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Mucor miehei lipase, Lipozyme, has been found to be vulnerable to temperature-induced deactivation. This thermodeactivation is further aggravated by exposure to butanol. The deleterious effect of the latter on the lipase is a function of its concentration in the aqueous phase containing the enzyme. At 50 degrees C, it was found that a crucial butanol concentration (0.81 M) exists, up to which there is only a slight decrease (25%) in lipase activity. Beyond this, the fall in activity is rapid, with total deactivation at 0.99 M concentration. The deactivation kinetics of the lipase are modeled using a series-type mechanism.

引用

页码：373 / 380

页数：8

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