IDENTIFICATION OF THE BPHA4 GENE ENCODING FERREDOXIN REDUCTASE INVOLVED IN BIPHENYL AND POLYCHLORINATED BIPHENYL DEGRADATION IN PSEUDOMONAS SP STRAIN KKS102

被引：54

作者：

KIKUCHI, Y ^{[1
]}

NAGATA, Y ^{[1
]}

HINATA, M ^{[1
]}

KIMBARA, K ^{[1
]}

FUKUDA, M ^{[1
]}

YANO, K ^{[1
]}

TAKAGI, M ^{[1
]}

机构：

[1] NAGAOKA UNIV TECHNOL,DEPT BIOENGN,NAGAOKA,NIIGATA 94021,JAPAN

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 06期

关键词：

D O I：

10.1128/jb.176.6.1689-1694.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nucleotide sequence of the downstream region of the bph operon from Pseudomonas sp. strain KKS102 was determined. Two open reading frames (ORF1 and ORF2) were found in this region, and the deduced amino acid sequence of ORF2 showed homology with the sequences of four ferredoxin reductases of dioxygenase systems. When this region was inserted just upstream of the bph operon, which does not contain a gene encoding ferredoxin reductase, biphenyl dioxygenase activity uas detected. The 24- and 44-kDa polypeptides predicted from the two open reading frames were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Crude extract which contained the products of ORF2 and bhpA1A2A3 showed cytochrome c reduction activity. These data clearly suggest that ORF2 encodes ferredoxin reductase. The deduced amino acid sequence of ORF1 does not show significant homology with the sequences of any other proteins in the SWISS-PROT data bank, and the function of ORF1 is unknown.

引用

页码：1689 / 1694

页数：6

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