GROWTH-STIMULATION OF TREPONEMA-DENTICOLA BY PERIODONTAL MICROORGANISMS

被引：25

作者：

TERSTEEG, PF ^{[1
]}

VANDERHOEVEN, JS ^{[1
]}

机构：

[1] CATHOLIC UNIV NIJMEGEN,POB 9101,6500 HB NIJMEGEN,NETHERLANDS

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 1990年 / 57卷 / 02期

关键词：

Bacteroides intermedius; Eubacterium nodatum; Fusobacterium nucleatum; serum; Treponema denticola; Veillonella parvula;

D O I：

10.1007/BF00403156

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Previous experiments have indicated that enrichment of subgingival plaque in human serum can lead to the accumulation of Treponema denticola. T. denticola depends on bacterial interactions for its growth in serum. Aim of the present study was to identify specific microorganisms involved in the growth stimulation of T. denticola. To this end, strains isolated from previous plaque enrichment cultures were tested for growth stimulation in co-cultures with T. denticola. In addition, growth of T. denticola was tested in culture filtrates of the same strains, Bacteroides intermedius, Eubacterium nodatum, Veillonella parvula and Fusobacterium nucleatum were found to enhance growth of T. denticola in co-cultures. A continuous co-culture of T. denticola, F. nucleatum and B. intermedius in human serum gave very high levels of T. denticola, up to 3.109 ml-1. Mechanisms involved in growth stimulation may include the ability of B. intermedius and E. nodatum to cleave the protein-core of serum (glyco-)proteins, making these molecules accessible for degradation by T. denticola. In addition, E. nodatum was found to produce a low-molecular weight growth-factor for T. denticola, that was heat-stable and acid as well as alkaline resistant. V. parvula may provide peptidase activities complementary to those of T. denticola. The nature of the growth enhancing activity of F. nucleatum is yet unknown. The data support the dependency of T. denticola on other bacterial species for growth in the periodontal pocket. © 1990 Kluwer Academic Publishers.

引用

页码：63 / 70

页数：8

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