FLUORESCENT DERIVATIVES OF POLYSACCHARIDE DIALDEHYDE AS SUBSTRATES FOR GLUCANASES

被引：12

作者：

KOBAYASHI, M ^{[1
]}

URAYAMA, T ^{[1
]}

ICHISHIMA, E ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC AGR,DEPT AGR CHEM,SENDAI,MIYAGI 981,JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1990年 / 54卷 / 07期

关键词：

D O I：

10.1080/00021369.1990.10870203

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A simple and sensitive assay method for glucanase activity was established using fluorescent polysaccharide substrates. Periodate oxidized a-glucans having dialdehyde were covalently attached to the fluorescent reagent via Schiff base formation followed by NaBH4 reduction. Ethylenediami-nonaphthalene (EDAN) was effective to produce a stable and highly fluorescent polysaccharide. ED AN derivatives of glycogen and dextran dialdehyde were useful substrates for Taka-amylase A and endodextranase, respectively. Two cellulases were shown to release water-soluble fluorescent products from the EDAN derivative of cellulose powder. Moreover, the action of the exo type of enzyme such as glucoamylase was readily distinguished from that of the endo type of enzyme because the attached EDAN prevented the attack of exo-enzyme. © 1990 by the Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

引用

页码：1711 / 1718

页数：8