EFFECT OF NADPH OXIDASE INHIBITION ON ENDOTHELIAL-CELL ELAM-1 MESSENGER-RNA EXPRESSION

被引：44

作者：

SUZUKI, Y

WANG, WZ

VU, TH

RAFFIN, TA

机构：

[1] STANFORD UNIV,MED CTR,DIV PULM & CRIT CARE MED,ROOM H3151,STANFORD,CA 94305

[2] VET AFFAIRS MED CTR,MED SERV,PALO ALTO,CA 94304

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)80029-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neutrophil adherence to endothelium is partially mediated by the expression of endothelial leukocyte adhesion molecule-1 (ELAM-1) on endothelial cells activated by agents such as lipopolysaccharide (LPS) and phorbol myristate acetate (PMA). To elucidate molecular mechanisms involved in the induction of ELAM-1 on endothelial cells, we investigated the effect of the NADPH oxidase inhibitor, apocynin (4-hydroxy-3-methoxyacetophenone), on ELAM-1 mRNA expression in human umbilical vein endothelial cells (HUVEC) by Northern blot analysis. Apocynin downregulated both LPS- and PMA-induced ELAM-1 mRNA expression in a dosedependent manner. Our results suggest NADPH oxidase might play a key role in ELAM-1 mRNA expression in HUVEC. © 1992 Academic Press, Inc.

引用

页码：1339 / 1343

页数：5

共 22 条

[21] AN NF KAPPA-B-LIKE FACTOR IS ESSENTIAL BUT NOT SUFFICIENT FOR CYTOKINE INDUCTION OF ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE 1 (ELAM-1) GENE-TRANSCRIPTION
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[22] MEASUREMENT OF ENDOTHELIAL-CELL FREE-RADICAL GENERATION - EVIDENCE FOR A CENTRAL MECHANISM OF FREE-RADICAL INJURY IN POSTISCHEMIC TISSUES
ZWEIER, JL
KUPPUSAMY, P
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[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (11) : 4046 - 4050

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