P-31-NMR STUDIES OF THE SOLUTION STRUCTURE AND DYNAMICS OF NUCLEOSOMES AND DNA

被引：66

作者：

KLEVAN, L ^{[1
]}

ARMITAGE, IM ^{[1
]}

CROTHERS, DM ^{[1
]}

机构：

[1] YALE UNIV,DEPT MOLEC BIOPHYS & BIOCHEM,NEW HAVEN,CT 06520

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 04期

关键词：

D O I：

10.1093/nar/6.4.1607

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

31P NMR studies of 140 base pair DNA fragments in nucleosomes and free in solution show no detectable change in the internucleotide 31p chemical shift or linewidth when DNA is packaged into nucleosomes. Measurements of 31p spin-lattice relaxation times T1 and 31p-{H} nuclear Overhauser enhancements revealed internal motion with a correlation time of about 4 × 10-10 sec in double helical DNA, both free in solution and bound to nucleosomal core proteins. This result implies greater dynamic mobility in double helical DNA than has previously been supposed. © 1979 Information Retrieval Limited.

引用

页码：1607 / 1616

页数：10

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