CLONING BY DIFFERENTIAL SCREENING OF A XENOPUS CDNA THAT ENCODES A KINESIN-RELATED PROTEIN

被引：142

作者：

LEGUELLEC, R ^{[1
]}

PARIS, J ^{[1
]}

COUTURIER, A ^{[1
]}

ROGHI, C ^{[1
]}

PHILIPPE, M ^{[1
]}

机构：

[1] UNIV RENNES 1, BIOL & GENET DEV LAB,CNRS,URA 256,CAMPUS BEAULIEU, F-35042 RENNES, FRANCE

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1991年 / 11卷 / 06期

关键词：

D O I：

10.1128/MCB.11.6.3395

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By differential screening of a Xenopus egg cDNA library, we selected nine clones (Eg1 to Eg9) corresponding to mRNAs which are deadenylated and released from polysomes soon after fertilization. The sequence of one of these clones (Eg5) revealed that the corresponding protein has the characteristic features of a kinesin-related protein. More specifically, Eg5 was found to be nearly 30% identical to a kinesin-related protein encoded by bimc, a gene involved in nuclear division in Aspergillus nidulans.

引用

页码：3395 / 3398

页数：4

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