IMMOBILIZATION OF DNA FRAGMENTS BY LANGMUIR - BLODGETT TECHNIQUE

DNA fragments can be immobilized by Langmuir — Blodgett films of 1-hexadecylamine. The inclusion in these LB films of 5'-GTAAAACGACGGCCAGT-3' was proved by a radioactive technique. An X-ray diffraction study proved an overall lamellar ordering of the samples and suggested a structural organization in which layers of oligonucleotide molecules are sandwiched between two monolayers of 1-hexadecylamine. These monolayers are likely to turn their amino groups surfaces to the oligonucleotide molecules as to screen their anionic charges. © 1992, Taylor & Francis Group, LLC. All rights reserved.