CYCLIC AMP-DEPENDENT REGULATION OF P-TYPE CALCIUM CHANNELS EXPRESSED IN XENOPUS-OOCYTES

被引：21

作者：

FOURNIER, F ^{[1
]}

BOURINET, E ^{[1
]}

NARGEOT, J ^{[1
]}

CHARNET, P ^{[1
]}

机构：

[1] CRBM,CNRS,UPR 9008,INSERM,U249,1919 ROUTE MENDE,BP 5051,F-34033 MONTPELLIER,FRANCE

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1993年 / 423卷 / 3-4期

关键词：

RAT CEREBELLUM; PHOSPHORYLATION; HYBRID ARREST; PROTEIN KINASE-A;

D O I：

10.1007/BF00374391

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Xenopus oocytes injected with rat cerebellum mRNA, express voltage-dependent calcium channels (VDCC). These were identified as P-type Ca2+ channels by their insensitivity to dihydropyridines and omega-conotoxin and by their blockade by Agelenopsis aperta venom (containing the funnel-web spider toxins: FIX and omega-Aga-IV-A). Coinjection of cerebellar mRNA and antisense oligonucleotide complementary to the dihydropyridine-resistant brain Ca2+ channel, named BI [Mori Y. et al. (1991) Nature 350:398-402] or rbA [Starr T. V. B. et al. (1991) Proc Natl Acad Sci USA 88: 5621-5625], strongly reduced the expressed Ba2+ current suggesting that these clones encode a P-type VDCC. The macroscopic Ca2+ channel activity was increased by direct intraoocyte injection of cAMP. This increase in current amplitude was concomitant with a slowing of current inactivation, and was attributed to activation of protein kinase A, since it could be antagonized by a peptidic inhibitor of this enzyme. Positive regulation of P-type VDCC could be of importance in Purkinje neurons and motor nerve terminals where this channel is predominant.

引用

页码：173 / 180

页数：8

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