STRUCTURAL INVESTIGATION OF THE CAPSULAR POLYSACCHARIDE OF ESCHERICHIA-COLI O101-K103-H- USING BACTERIOPHAGE DEGRADATION AND NMR-SPECTROSCOPY

被引：7

作者：

GRUE, MR ^{[1
]}

PAROLIS, H ^{[1
]}

PAROLIS, LAS ^{[1
]}

机构：

[1] RHODES UNIV,SCH PHARMACEUT SCI,GRAHAMSTOWN 6140,SOUTH AFRICA

来源：

CARBOHYDRATE RESEARCH | 1994年 / 262卷 / 02期

关键词：

ESCHERICHIA-COLI; CAPSULAR POLYSACCHARIDE; BACTERIOPHAGE DEPOLYMERIZATION; NMR SPECTROSCOPY; PYRUVATE;

D O I：

10.1016/0008-6215(94)84187-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NMR spectroscopy was performed on the depyruvated capsular antigen of E. coli K103 and on the oligosaccharide obtained by depolymerisation of the native polysaccharide with a viral-borne endoglycanase. This capsular polysaccharide is the only one to be co-expressed with O group 101 and joins a small group of unusual capsular polysaccharides which possess pyruvic acid as the only acidic function. The primary structure was shown to be composed of the repeating unit: [GRAPHICS]

引用

页码：311 / 322

页数：12