METABOLISM OF A NOVEL ANTITUMOR AGENT, CRISNATOL, BY A HUMAN HEPATOMA-CELL LINE, HEP-G2, AND HEPATIC MICROSOMES - CHARACTERIZATION OF METABOLITES

被引：13

作者：

PATEL, DK ^{[1
]}

SHOCKCOR, JP ^{[1
]}

CHANG, SY ^{[1
]}

SIGEL, CW ^{[1
]}

HUBER, BE ^{[1
]}

机构：

[1] BURROUGHS WELLCOME CO,RES TRIANGLE PK,NC 27709

来源：

BIOCHEMICAL PHARMACOLOGY | 1991年 / 42卷 / 02期

关键词：

D O I：

10.1016/0006-2952(91)90721-G

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Metabolism of the anticancer agent crisnatol was investigated using a human hepatoma cell line, Hep G2, and human liver microsomes. Crisnatol was metabolized extensively by both systems. The TLC/autoradiographic analysis showed that the crisnatol metabolite profile was similar for both systems and the major metabolites were shown to have structural characteristics similar to those formed by the rat. The Hep G2 cells formed three isomeric dihydrodiols; one of these has been identified by GC/MS and H-1-NMR as the crisnatol 1,2-dihydrodiol. Human liver microsomes also formed two isomeric dihydrodiols with 1,2-dihydrodiol as the major isomer and, in addition, produced 1-hydroxycrisnatol. Crisnatol concentrations of 1.3-mu-g/mL completely inhibited the replication of Hep G2 cells as measured by thymidine incorporation and cell growth kinetics and, at this concentration, cell viability decreased by only 35% as determined by vital staining of cells using neutral red dye.

引用

页码：337 / 346

页数：10

共 38 条

[31] GLUCURONIDATION OF 3'-AZIDO-3'-DEOXYTHYMIDINE - HUMAN AND RAT ENZYME SPECIFICITY [J].