GROWTH-INHIBITION AND APOPTOSIS IN HUMAN NEUROBLASTOMA SK-N-SH CELLS INDUCED BY HYPERICIN, A POTENT INHIBITOR OF PROTEIN-KINASE-C

被引：46

作者：

ZHANG, W

LAWA, RE

HINTONA, DR

SU, YZ

COULDWELL, WT

机构：

[1] UNIV SO CALIF,SCH MED,DEPT NEUROL SURG,LOS ANGELES,CA 90033

[2] UNIV SO CALIF,SCH MED,DEPT MED,DIV ENDOCRINOL DIABET & HYPERTENS,LOS ANGELES,CA 90033

来源：

CANCER LETTERS | 1995年 / 96卷 / 01期

关键词：

NEUROBLASTOMA; HYPERICIN; APOPTOSIS; PROTEIN KINASE C;

D O I：

10.1016/0304-3835(95)03914-I

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The effect of hypericin, an antiviral agent and inhibitor of protein kinase C (PKC), on cell proliferation and programmed death was investigated in the human neuroblastoma cell line SK-N-SH. Hypericin induced significant growth inhibition in a dose-dependent manner demonstrated by a microculture tetrazolium (MTT) assay. DNA isolated from cells treated with hypericin at concentrations over 1 mu M exhibited a 'ladder' pattern of oligonucleosome-sized fragments characteristic of apoptosis. Similarly, treatment of the cells with the PKC inhibitors staurosporine, tamoxifen or phorbol ester PMA for 72 h also resulted in apoptosis, suggesting that hypericin may be triggering an apoptotic signal in neuroblastoma cells, which at least in part may be mediated by the inhibition of PKC.

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页码：31 / 35

页数：5

相关论文

共 21 条

[11] ANTIVIRAL ACTIVITIES OF HYPERICIN [J].

HUDSON, JB ;

LOPEZBAZZOCCHI, I ;

TOWERS, GHN .

ANTIVIRAL RESEARCH, 1991, 15 (02) :101-112

[12]

JALAVA A, 1992, FEBS LETT, V2, P114

[13] CALPHOSTIN C (UCN-1028C), A NOVEL MICROBIAL COMPOUND, IS A HIGHLY POTENT AND SPECIFIC INHIBITOR OF PROTEIN KINASE-C [J].

KOBAYASHI, E ;

NAKANO, H ;

MORIMOTO, M ;

TAMAOKI, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 159 (02) :548-553

[14] STUDIES OF THE MECHANISMS OF ACTION OF THE ANTIRETROVIRAL AGENTS HYPERICIN AND PSEUDOHYPERICIN [J].

LAVIE, G ;

VALENTINE, F ;

LEVIN, B ;

MAZUR, Y ;

GALLO, G ;

LAVIE, D ;

WEINER, D ;

MERUELO, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (15) :5963-5967

[15] DIFFERENTIAL EXPRESSION AND SUBCELLULAR-LOCALIZATION OF PROTEIN-KINASE-C ALPHA-ISOFORM, BETA-ISOFORM, GAMMA-ISOFORM, DELTA-ISOFORM AND EPSILON-ISOFORM IN SH-SY5Y NEUROBLASTOMA-CELLS - MODIFICATIONS DURING DIFFERENTIATION [J].

LELI, U ;

SHEA, TB ;

CATALDO, A ;

HAUSER, G ;

GRYNSPAN, F ;

BEERMANN, ML ;

LIEPKALNS, VA ;

NIXON, RA ;

PARKER, PJ .

JOURNAL OF NEUROCHEMISTRY, 1993, 60 (01) :289-298

[16] PHOTODYNAMIC INACTIVATION OF INFECTIVITY OF HUMAN-IMMUNODEFICIENCY-VIRUS AND OTHER ENVELOPED VIRUSES USING HYPERICIN AND ROSE-BENGAL - INHIBITION OF FUSION AND SYNCYTIA FORMATION [J].

LENARD, J ;

RABSON, A ;

VANDEROEF, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (01) :158-162

[17] THERAPEUTIC AGENTS WITH DRAMATIC ANTIRETROVIRAL ACTIVITY AND LITTLE TOXICITY AT EFFECTIVE DOSES - AROMATIC POLYCYCLIC DIONES HYPERICIN AND PSEUDOHYPERICIN [J].

MERUELO, D ;

LAVIE, G ;

LAVIE, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (14) :5230-5234

[18] THE FAMILY OF PROTEIN KINASE-C FOR SIGNAL TRANSDUCTION [J].

NISHIZUKA, Y .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1989, 262 (13) :1826-1833

[19]

TAKAHASHI I, 1989, BIOCHEM BIOPH RES CO, V157, P755

[20]

1991, MINN MED, V74, P46