CHOLESTEROL OXIDASE - A POTENT INSECTICIDAL PROTEIN ACTIVE AGAINST BOLL-WEEVIL LARVAE

被引：115

作者：

PURCELL, JP ^{[1
]}

GREENPLATE, JT ^{[1
]}

JENNINGS, MG ^{[1
]}

RYERSE, JS ^{[1
]}

PERSHING, JC ^{[1
]}

SIMS, SR ^{[1
]}

PRINSEN, MJ ^{[1
]}

CORBIN, DR ^{[1
]}

TRAN, M ^{[1
]}

SAMMONS, RD ^{[1
]}

STONARD, RJ ^{[1
]}

机构：

[1] ST LOUIS UNIV, SCH MED, ST LOUIS, MO 63104 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.2409

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The discovery of proteins that control insects is critical for the continued growth of the agricultural biotechnology industry. A highly efficacious protein that killed boll weevil (Anthonomus grandis grandis Boheman) larvae was discovered in Streptomyces culture filtrates. The protein was identified as cholesterol oxidase (EC 1.1.3.6). Purified cholesterol oxidase was active against boll weevil larvae at a concentration (LC50 = 20.9 μg/ml) comparable to the bioactivity of Bacillus thuringiensis proteins against other insect pests. Histological studies demonstrated that cholesterol oxidase lysed the boll weevil midgut epithelium, suggesting that this is the primary mechanism of lethality. © 1993 Academic Press, Inc.

引用

页码：1406 / 1413

页数：8

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