CHROMATOGRAPHIC-SEPARATION OF UROPORPHYRIN-I AND UROPORPHYRIN-III OCTAMETHYL ESTERS

被引：24

作者：

BOMMER, JC ^{[1
]}

BURNHAM, BF ^{[1
]}

CARLSON, RE ^{[1
]}

DOLPHIN, D ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,TRIUMF,DEPT CHEM,VANCOUVER V6T 1W5,BC,CANADA

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90753-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Uroporphyrins I and III have been separated by high performance liquid chromatography on a microporasil column using n-heptane:glacial acetic acid:acetone:water (90:60:30:0.05 by volume). By using uro [3H]porphyrin I and uro[14C]porphyrin III it was shown that less than 2% cross contamination occurred during the chromatographic separation. The same system may be used to separate uro through protoporphyrins (as their esters) by simply increasing the acetone concentration. © 1979.

引用

页码：444 / 448

页数：5

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