IDENTIFICATION OF THE PROTEOLYTICALLY ACTIVATED FORM OF PROTEIN KINASE-C IN STIMULATED HUMAN NEUTROPHILS

被引：70

作者：

PONTREMOLI, S ^{[1
]}

MICHETTI, M ^{[1
]}

MELLONI, E ^{[1
]}

SPARATORE, B ^{[1
]}

SALAMINO, F ^{[1
]}

HORECKER, BL ^{[1
]}

机构：

[1] CORNELL UNIV,MED CTR,COLL MED,NEW YORK,NY 10021

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 10期

关键词：

cell activation; protein kinase C down-regulation; protein kinase M;

D O I：

10.1073/pnas.87.10.3705

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The proteolytically activated form of protein kinase C has been identified in human neutrophils by using a monoclonal antibody that recognizes both the native kinase and the catalytically active proteolytic fragment (protein kinase M). Stimulation with fMet-Leu-Phe results in the conversion of ≃ 30% of native protein kinase C to protein kinase M, with little evidence of further degradation. Stimulation with phorbol 12-myristate 13-acetate, on the other hand, causes only a transient formation of protein kinase M, with complete loss of total kinase activity. These differences are related to the differences in biochemical responses, reported earlier, in neutrophils exposed to these two activators.

引用

页码：3705 / 3707

页数：3

共 26 条

[11]

KISHIMOTO A, 1983, J BIOL CHEM, V258, P1156

[12]

KRAFT AS, 1982, J BIOL CHEM, V257, P13193

[13] ACTIVATION OF THE RESPIRATORY BURST ENZYME IN HUMAN POLYMORPHONUCLEAR LEUKOCYTES BY CHEMOATTRACTANTS AND OTHER SOLUBLE STIMULI - EVIDENCE THAT THE SAME OXIDASE IS ACTIVATED BY DIFFERENT TRANSDUCTIONAL MECHANISMS [J].