SIMULTANEOUS ENANTIOSELECTIVE DETERMINATION AND QUANTIFICATION OF DIMETHINDENE AND ITS METABOLITE N-DEMETHYL-DIMETHINDENE IN HUMAN URINE USING CYCLODEXTRINS AS CHIRAL ADDITIVES IN CAPILLARY ELECTROPHORESIS

被引：28

作者：

HEUERMANN, M ^{[1
]}

BLASCHKE, G ^{[1
]}

机构：

[1] UNIV MUNSTER,DEPT PHARMACEUT CHEM,D-48149 MUNSTER,GERMANY

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1994年 / 12卷 / 06期

关键词：

DIMETHINDENE; URINE SAMPLES; CAPILLARY ELECTROPHORESIS; CYCLODEXTRINS; CHIRAL;

D O I：

10.1016/0731-7085(93)E0024-H

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary electrophoresis (CE) has been used for the chiral resolution of the basic racemic drug dimethindene (Fenistil(R)) and its metabolite N-demethyl-dimethindene. After oral administration of 4 mg dimethindene maleate the enantioselective excretion in human urine was determined by capillary electrophoresis using a 50 mM phosphate run buffer (pH 3.3) containing 30 mM hydroxypropyl-beta-cyclodextrin. The determination of very low concentrations of dimethindene and N-demethyl-dimethindene in human urine was possible using sample stacking conditions to increase the amount of analyte in the capillary with good peak forms and resolutions. The detection limit of the method was approximately 1 ng ml(-1) urine for each compound.

引用

页码：753 / 760

页数：8

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